Establishment of a duplex SYBR green I-based real-time polymerase chain reaction assay for the rapid detection of canine circovirus and canine astrovirus
نویسندگان
چکیده
منابع مشابه
Development of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus
Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
متن کاملCharacterization of Iranian Isolates of Canine Parvovirus in Fecal Samples Using Polymerase Chain Reaction Assay
Despite the widespread prevalence of canine parvovirus disease (CPV) in Iranian dog population,molecular diagnosis of CPV variants, and investigation of the trends of its genetic changes is a new effort. Inthis study 50 samples from dogs suspicious of infection with clinical signs of diarrhea and vomiting, and 25samples from dogs suspected of infection with general symptoms su...
متن کاملRapid Detection of Porcine Circovirus Type 2 by TaqMan-based Real-time Polymerase Chain Reaction Assays
PCV2 is the primary causative agent of porcine circovirus-associated disease (PCVAD). In this study, a TaqMan-based real-time polymerase chain reaction (PCR) assay targeting ORF2 gene of PCV2 was developed and their sensitivities and specificities were investigated. The results indicated that the standard curve had a wide dynamic range (102-107copies/μL) with a linear correlation (R2) of 0.997 ...
متن کاملDetection and differentiation of wild-type and vaccine strains of canine distemper virus by a duplex reverse transcription polymerase chain reaction
Canine distemper virus (CDV) is the cause of canine distemper (CD) which is a severe and highly contagious disease in dogs. In the present study, a duplex reverse transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of CDV. Four primers were designed to detect and discriminate the two viruses by generating 6...
متن کاملA duplex SYBR Green I real-time quantitative PCR assay for detecting Escherichia coli O157:H7.
PCR and hybridization assays are widely used for the detection and identification of Escherichia coli serogroups and serotypes. We used these techniques for the detection of E. coli O157:H7, a dominant serogroup among E. coli strains that are considered major public health problems worldwide. We developed a quantitative PCR assay using SYBR Green I, based on the published sequences of the rfbE ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular and Cellular Probes
سال: 2020
ISSN: 0890-8508
DOI: 10.1016/j.mcp.2020.101666